Purexa™ MCP
for Supercoiled pDNA Polishing
Purexa™ MCP is the first commercially available membrane chromatography product for the polishing of supercoiled pDNA. It offers up to 10x productivity compared to traditional resins.
How Purexa™ MCP Works
Purexa™ MCP is the cornerstone of our pDNA purification offering and is able to successfully separate supercoiled pDNA from impurities such as open circular pDNA. Utilizing hydrophobic interactions, our porous membranes are functionalized for better purification.
Superior productivity:
up to 10x with Purexa™ MCP
Higher dynamic binding capacity
Faster cycle times
Consistent performance through linear scale up
And easier setup and breakdown
Figure 2: Size Comparison: Biologics vs. Pores compares the sizes of these common biologics to the sizes of both resin and membrane pores.
Higher binding capacity with similar recovery...
Figure 2. The 85% recovery in eluate is comparable to resin products.
... with up to 20x faster flow rates ...
Figure 3. Purexa™ MCP is able to function at 22 times faster flow rates compared to tradition resin products with 10 times higher binding capacity.
... and consistent performance across multiple pDNA sizes.
Figure 4. In pDNA purification with Purexa™ MCP, dynamic binding capacity (DBC) is not inhibited by base pair size at a flow rate of 40 CV/mL without any clogging or pressure issues.
Purilogics’ membranes can purify
and are not limited to these common biologics :
The Purexa™ product line has pore sizes ranging from 0.3µm to 5 µm. With a much larger pore size, the target molecule is easily able to bind to the membrane at a high capacity, regardless of the size. Thus, Purilogics’ membrane chromatography products can be applied for the purification of many sizes of biologics while also maintaining and a rapid flow rate and high binding capacity.
Monoclonal Antibody
mRNA
Adeno-associated virus (AAV)
Lentivirus/Lentiviral vectors (LV)
Plasmid DNA
Oligo nucleotides; Nucleotides/Nucleosides